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Direct gene transfer methods can also called as vector free methods because no living vector used to transfer gene to plant cells. Many physical and mechanical methods were developed that facilitate the entry of this foreign DNA into plant cells. This aspect of B Direct Gene Transfer Dav University plays a vital role in practical applications.

Furthermore, independent of the hostrange problem it was a challenge to achieve gene transfer directly. The approaches which have been successful so far include "direct gene transfer" (including elec troporation), "liposome fusion", "spheroplast fusion" and "microin jection". This aspect of B Direct Gene Transfer Dav University plays a vital role in practical applications.

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Many cells are so sensitive that the chemical method cannot be applied whereas some cells die during the treatment. This method is not perfect because many treated cells do not contain any transfer DNA. Sometimes the foreign DNA is degraded in the cytoplasm before reaching the nucleus. This aspect of B Direct Gene Transfer Dav University plays a vital role in practical applications.

Furthermore, direct gene transfer PDF - SlideShare. This aspect of B Direct Gene Transfer Dav University plays a vital role in practical applications.

Moreover, independent of the hostrange problem it was a challenge to achieve gene transfer directly. The approaches which have been successful so far include "direct gene transfer" (including elec troporation), "liposome fusion", "spheroplast fusion" and "microin jection". This aspect of B Direct Gene Transfer Dav University plays a vital role in practical applications.

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